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Image Processing and Analysis @EMBL

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Downloads

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Type Name Platform Author Last UpDate
Analysis Frap Analysis ver 9 IgorPro Kota Miura 10-03-03
Spindle FRAP/FLIP analyze ImageJ Kota Miura 08-08-12
Kymoquant ImageJ Kota Miura 10-03-03
– automated analysis of kymograph
ParticleTracker ImageJ Kota Miura 10-11-19
Stack Tools Time Series color coder ImageJ Kota Miura 08-07-23
Running 3D-Projector ImageJ Kota Miura 08-08-29
Stack Manager ImageJ Kota Miura 06-06-08
Bleach Corrector ImageJ Kota Miura 12-04-23
Visualization 3D track viewer ImageJ Kota Miura 12-04-23
File Conversion Perkin Elmer Tiff Series to 3D stacks for 3D Manual Tracking ImageJ Kota Miura 07-06-05
DeltaVision Format Header Reader for Collapsed .dv files ImageJ Kota Miura 07-06-13
Software Linker Export Hyperstack to Imaris ImageJ/Imaris Kota Miura 10-08-06
Coding Tools Notepad++ Syntax High-Lighter for ImageJ Macro Notepad++ Kota Miura 10-11-00
Vim Syntax High-Lighter for ImageJ Macro VIM Kota Miura 10-11-29
Drop Script ImageJ Kota Miura 12-02-19

Un-Documented Codes

Some of the codes, still not documented.

Scripts

  • kymotrackPlotter.py (2012/04/25 16:07)

    Plots Bory project tracking results onto 3D-kymograph.
     noteFilepath and dimension of the kymograph should be adjusted manually.

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  • 3dprojection.py (2012/04/25 16:05)

    4D stack kymograph (by maximum projection)
    requires a 3D timeseries (4D hyperstack) to be opened.
    Outputs three 2D kymographs (xz, yz, xy projections to y, x, z axes, respectively)
    ...written in conjunction with Bory's project on chromosome locus distance dynamics
    note: z is not scaled by XY scale, so the width of xy projection image
    # should be adjusted by resizing according to z factor.

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  • bleachCorHM.js (2012/04/25 13:58)

    reuires: Bleach Correction plugin (or Fiji)
    Does bleach correction using Histogram matching method in headless environment.
    e.g.
    fiji -headless bleachCorHM.js

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  • Correct_3D_drift_headless.py (2012/04/25 13:49)

    Requires: Fiji
    Headless version of Correct 3D drift written by Robert Bryson-Richardson and Albert Cardona. For use from command line, such as 
    ./fiji Correct_3D_drift_headless.py file_path destination_folder
    arg1 is the full path to the file to be corrected, and the destination folder is where the corrected stack is saved.

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  • 3Ddots.js (2012/04/25 13:44)

    script for 3D spot detection using ParticleTracker3D and creating a stack with same size of the current stack, and plot xyz dots listed in results window (the results of ParticleTracker3D plugin).

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  • zprojSUM_LIF_headless.py (2012/04/25 13:43)

    Z-sum-projection script for LIF file.
    works from command line, intended to be used in server - data storage.
    Useful for processing huge data sets without occupying your desktop.

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  • zproj_TifSeries_headless.py (2012/04/25 13:41)

    Z-max-projection script for Tiff series.
    works from command line, intended to be used in server - data storage.
    Useful for processing huge data sets without occupying your desktop.

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  • zproj_LIF_headless.py (2012/04/25 13:40)

    Z-max-projection script for LIF file.
    works from command line, intended to be used in server - data storage.
    Useful for processing huge data sets without occupying your desktop. For converting stacks within .lif file to z-projected tiff stacks in file server. All files within the directory given as the first argument will be processed.
    e.g.
    fiji headlessZprojector.py /g/cmci/test/

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  • affinetransform3Dv3.js (2012/04/25 13:14)

    Loads csv files with feature points and transforms 3D stack.

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  • K_3Dprofile.js (2012/04/25 13:13)

    Requires: Fiji
    ...or ImageJ with Apache commons math.
    3D intensity profiler, from a list of coordinates (start point and end point) in csv file.

    workflow: prepare a tab-delimited data file with a pair of 3D coordinates per line(6 numbers per line).
    Run this script, and in dialog window choose the file, then 3D intensity profile in the top-image window will be calculated and shown in the  Results table.

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  • 3DdiscV3.js (2012/04/25 13:13)

    Requires: Fiji

    3D disc generator using commons Vector3D A part of 3D intensity profiler.

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  • Dot3Danalysis.py (2012/04/25 13:10)

    Requires: ImageJ, DotDetector3D plugin (ask Kota).
    Dot detection by segmentation of ch1 and measures ch2 with a bit wider area.
    Dot coordinates are detected in 3D. Multiple dots being too close to each other are omitted from measurement since with the application this code was written, focal depth is thick. 3D intensity measurement is currently omitted.

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  • 3Ddots.js (2012/01/26 12:23)

    script for 3D spot detection using ParticleTracker3D and creating a stack with same size of the current stack, and plot xyz dots listed in results window (the results of ParticleTracker3D plugin).

    Tags:

  • K_LIFfileImporter.ijm (2012/01/26 11:56)

    ImageJ macro

    for opening specific channel in LIF dataset.

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  • highpassFilter.py (2012/01/26 11:47)

    High-pass filter using scipy and numpy. 

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  • batchXls2csv.py (2012/01/26 11:45)

    Batch conversion of excel file to csv file

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  • loadVecandMayaviPlot.py (2012/01/26 11:43)

    Loads vectors (csv file) and plots as a 3D vector field in mayavi

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  • openTiffAsVolume.py (2012/01/26 11:41)

    Requires: Python (wrote with 2.6)
    + additional modules Mayavi, tiffile, tkFileDialog
     
    Loads a tiff stack and display it in mayavi.

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