Snow -> Rain in Heidelberg
Since morning it was heavily snowing but then from 14:00, snow turned into rain. If road surface will be frozen, famous disaster down the Steigerweg is expected (crashed cars, abandoned cars..)
(1) Bleaching Correction macro
A year ago or so, Jens Rietfdorf (now in Basel) made a macro to correct for Bleaching of fluorescence of image series. Julien Colombelli asked me for different reason, some darkening of the image due to whatever camera setting or so, but the same macro should be working for this and also for all other similar decay of signal. This imageJ macro is downloadable from EAMNET website.
(2) FRAP
Fatima Verissimo asked me about FRAP fitting. Before doing experiment, go through chapter 2 in FRAP maual to see what kind of data (what you should measure out of FRAP image sequences) is required. I discussed with fatima about how to do FRAP analysis when the bleached ROI moves around after bleaching. This is a challenging task. The best way would be to label the target structure with a probe with a different fl. wavelength from the probe one is using for the FRAP. This none-FRAPped fl. signal would then be used for tracking the position of the structure. If the second probe is not possible... then that will be something to think about how to do.
(3) Going through Heiko's paper on Cholesterol. Great data, great paper. The project is also techincally rich FRAP, fl. kinetic studies, Modeling etc etc.
(4) 2D cross-correlation fixing. Somthing is wrong, probably small mistakes in back calculating 1D array to 2D arary (xy coordinates).
(1) Bleaching Correction macro
A year ago or so, Jens Rietfdorf (now in Basel) made a macro to correct for Bleaching of fluorescence of image series. Julien Colombelli asked me for different reason, some darkening of the image due to whatever camera setting or so, but the same macro should be working for this and also for all other similar decay of signal. This imageJ macro is downloadable from EAMNET website.
(2) FRAP
Fatima Verissimo asked me about FRAP fitting. Before doing experiment, go through chapter 2 in FRAP maual to see what kind of data (what you should measure out of FRAP image sequences) is required. I discussed with fatima about how to do FRAP analysis when the bleached ROI moves around after bleaching. This is a challenging task. The best way would be to label the target structure with a probe with a different fl. wavelength from the probe one is using for the FRAP. This none-FRAPped fl. signal would then be used for tracking the position of the structure. If the second probe is not possible... then that will be something to think about how to do.
(3) Going through Heiko's paper on Cholesterol. Great data, great paper. The project is also techincally rich FRAP, fl. kinetic studies, Modeling etc etc.
(4) 2D cross-correlation fixing. Somthing is wrong, probably small mistakes in back calculating 1D array to 2D arary (xy coordinates).
posted by Kota at 2:34 PM
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