spot counting

I was thinking about how to automatically count the number of ERES within cell. One way I initially thought was to do it by Gaussian fitting. Scan through 2D plane and find positions where Gaussian fitting works well (probably evaluate by chi-sq or Q value). Another way is simple particle analysis after processing image by (1) anisotropic diffusion (2) background subtraction (3) then do particle analysis.

wrote a Igor procedure to calculate average distances between ERES from a list of xy coordinates. (K_distancestats(xpos, ypos))