Tuesday, March 25, 2008

Frapcalc: V9c

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Update Note (ver9c)

Mark Kwaaitaal (Max Planck Institute for Plant Breeding Research) wrote me a email pointing out that he could not see drop-down menu in the Fitpanel. I searched for the cause of problem and this defect was due to a change from IgorPro ver5 to ver6. As stated in the notes for the ver6 release

Extended syntax for PopupMenu and ValDisplay value keyword: Previously, using the # prefix meant that the following text needed to be a literal quoted string. Now, the following text can be a string expression that will be evaluated at run time to obtain the actual string expression that needs to be executed each time the popup menu is used. In other words, there is a level of indirection. (link)

and communication in email list (Link), global variable cannot be used for the command popupmenu. Only quoted string or string expression function is allowed. For this reason, following line

PopupMenu popup1,value=# G_MethodList

was replaced to

PopupMenu popup1,value=# "G_MethodList"

Two such lines were replaced.

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Tuesday, October 30, 2007

FRAP analysis: Questions and Answers

For questions and comments, please feel free to post here in the comments section.

071030 ver9b
Stefan told me problem of negative Half Life value [s] for some cases, and I found out that when the data time range is too short and not reaching plateu, the calculation of HL results strange values. This is because HL in double exponential fitting is calculated by numerically interpolating the half max value.

To avoid this problem, estimation curve is not extrapolated that the HL becomes available even for the experiments with short time range. [now, still have problems to cope with "weighting" adjustments]

080325 ver9c
fixed popupmenu problem.
http://www.embl.org/cmci/weblog/2008/03/frapcalc-questions-and-answer-please.html

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