Snow -> Rain in Heidelberg

Since morning it was heavily snowing but then from 14:00, snow turned into rain. If road surface will be frozen, famous disaster down the Steigerweg is expected (crashed cars, abandoned cars..)

(1) Bleaching Correction macro
A year ago or so, Jens Rietfdorf (now in Basel) made a macro to correct for Bleaching of fluorescence of image series. Julien Colombelli asked me for different reason, some darkening of the image due to whatever camera setting or so, but the same macro should be working for this and also for all other similar decay of signal. This imageJ macro is downloadable from EAMNET website.

(2) FRAP
Fatima Verissimo asked me about FRAP fitting. Before doing experiment, go through chapter 2 in FRAP maual to see what kind of data (what you should measure out of FRAP image sequences) is required. I discussed with fatima about how to do FRAP analysis when the bleached ROI moves around after bleaching. This is a challenging task. The best way would be to label the target structure with a probe with a different fl. wavelength from the probe one is using for the FRAP. This none-FRAPped fl. signal would then be used for tracking the position of the structure. If the second probe is not possible... then that will be something to think about how to do.

(3) Going through Heiko's paper on Cholesterol. Great data, great paper. The project is also techincally rich FRAP, fl. kinetic studies, Modeling etc etc.

(4) 2D cross-correlation fixing. Somthing is wrong, probably small mistakes in back calculating 1D array to 2D arary (xy coordinates).

new papers

cortical flow related (Bray):

Nature Cell Biology 8, 216 - 226 (2006)
Published online: 26 February 2006; | doi:10.1038/ncb1367

Myosin II functions in actin-bundle turnover in neuronal growth cones
Nelson A. Medeiros, Dylan T. Burnette & Paul Forscher

Nature Cell Biology 8, 264 - 270 (2006)
Published online: 12 February 2006; | doi:10.1038/ncb1370

A microtubule-binding domain in dynactin increases dynein processivity by skating along microtubules

Tara L. Culver–Hanlon1, Stephanie A. Lex1, Andrew D. Stephens1, Nicholas J. Quintyne2, 3 &
Stephen J. King1

FRAP modeling::
Biophysical Journal 90:1878-1894 (2006)

Dissecting the Contribution of Diffusion and Interactions to the Mobility of Nuclear Proteins

Joël Beaudouin, Felipe Mora-Bermúdez, Thorsten Klee, Nathalie Daigle and Jan Ellenberg

Biophys. J. 2006 90: 2213-2220
Wei-hui Guo, Margo T. Frey, Nancy A. Burnham, and Yu-li Wang
Substrate Rigidity Regulates the Formation and Maintenance of Tissues

Heiko, Virginie, Darren

(1) Heiko paper's movie making. Restrictions on movies are very tight. I down-sized 250Mb movies to 1.5Mb, but it still seems to be TOO BIG! What a journal. (I don't name it)

(2) Virginie & Darren project.
Very interesting, vector field works very well.